Illumina HiSeq 2000

HiSeq 2000


Applications: DNA Sequencing, Gene Regulation Analysis, Sequencing-Based Transcriptome Analysis, SNP Discovery and Structural Variation Analysis, Cytogenetic Analysis, DNA-Protein Interaction Analysis (ChIP-Seq), Sequencing-Based Methylation Analysis, Small RNA Discovery and Analysis


The HiSeq 2000 sequencing system offers unprecedented output and a breakthrough user experience. Leveraging Illumina's proven and widely-adopted, reversible terminator-based sequencing by synthesis chemistry in combination with innovative engineering, HiSeq 2000 delivers the industry's highest sequencing output and fastest data generation rate. Human interaction design features and the easiest sequencing workflow set a new standard for simplicity and user experience.
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System Description

With innovative design features, HiSeq 2000 is the easiest to use next-generation sequencing system.

  • Flow cells are easily loaded on the vacuum-controlled loading dock.
  • Pre-configured, plug-and-play reagents sufficient for up to 200 cycles drop into racks in the machine's chiller compartment, requiring only two minutes of hands-on time.
  • A simple touch screen user interface, including on-screen, step-by-step instructions with embedded multimedia help, simplifies run setup.
  • Real-time progress indicators provide at-a-glance status, and remote monitoring allows a single user to check progress on multiple systems from any browser or internet-enabled phone.
  • HiSeq 2000 can be operated in single or dual flow cell mode, offering unmatched experimental flexibility and instrument scalability.
  • Independently-operable flow cells allow applications requiring different read lengths to run simultaneously.
  • Illumina's efficient and scalable data analysis solution for transforming billions of bases of raw sequencing data into publishable, biologically meaningful results is included.

 

HIGHLIGHTS

Unprecedented Output

Up to 200 Gb per run, 2 x 100 bp read length, up to 25 Gb per day, two billion paired-end reads/run.

Breakthrough User Experience

Pre-configured, plug-and-play reagents, simple flow cell loading, touch screen-enabled user interface for step-by-step run setup, and integrated paired-end fluidics.

Unmatched Cost-Effectiveness

In a single run, sequence two human genomes at ~30x coverage for less than $10,000 (USD) per genome, or perform 200 gene expression profiles for less than $200 per sample.

 

Sequence at a Scale Never Before Possible

HiSeq 2000 is the first commercially available sequencer to enable researchers to obtain ~30x coverage of two human genomes in a single run for under $10,000 (USD)* per sample.

 

HiSeq 2000 makes it possible for individual labs to take on the largest and most complex sequencing studies at the lowest cost. The ability to process larger numbers of samples and to decode larger and more complex genomes means that virtually any sequencing project is now within reach.

 

*Reagent costs at list price.

Upcoming Events

Advances in Genome Biology and Technology (AGBT)
February 24-27, 2010
Marco Island, FL

Workflow & Specs

HiSeq 2000 Preliminary Performance SpecificationsHiSeq 2000 Workflow

Options & Accessories

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HiSeq 2000 Kits

HiSeq Sequencing Kit (200 cycles)

HiSeq Sequencing Kit (200 cycles)

The HiSeq Sequencing Kit contains ready-to-load reagents for accurately determining the DNA sequence of each cluster on a flow cell using sequencing by synthesis technology on the HiSeq 2000 sequencing system.

Additional Details

FC-401-1001
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HiSeq 2000 Paired-End Cluster Generation Kit

HiSeq 2000 Paired-End Cluster Generation Kit

The HiSeq Paired-End Cluster Generation Kit provides reagents that bind samples to complementary adapter oligos on the flow cell. These enable copying of the DNA strand after the first sequencing run; the copied strand is used to sequence the other fragment end. cBot amplifies attached DNA fragments to clonal clusters of ~1000 copies each.

Additional Details

PE-401-1001
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HiSeq 2000 Paired-End Cluster Generation Kit

HiSeq 2000 Paired-End Cluster Generation Kit

The HiSeq Paired-End Cluster Generation Kit provides reagents that bind samples to complementary adapter oligos on the flow cell. These enable copying of the DNA strand after the first sequencing run; the copied strand is used to sequence the other fragment end. cBot amplifies attached DNA fragments to clonal clusters of ~1000 copies each.

Additional Details

PE-401-1001
ADD TO CART

Software

Illumina proudly offers system and analysis software designed to maximize your data quality and throughput level. Each instrument includes system control software designed for optimal performance. Data can be analyzed using Illumina's user-friendly GenomeStudio software or a third-party package.

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Support

Illumina's Customer Solutions Team is dedicated to providing you with the resources you need to support your research.  Scientists specializing in Illumina's technologies, scientific applications, software, and hardware systems are available even before your first system purchase.  Field Service Engineers expertly install and qualify your instrument to guarantee a fully functional system.  Field Application Scientists offer extensive training, enabling labs to quickly become proficient at generating industry-leading data quality at unparalleled throughput levels.  Technical Support Scientists are just a phone call or email away and can help you plan your project, diagnose a problem, and interpret results.

We strive to continuously exceed your expectations, so that you can maximize the impact of Illumina technology in your lab.  For more information, visit our Support section  


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